Site Directed Mutagenesis (SDM) Human Point mutation MDA-MB-231

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U20S

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - A2780

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U87

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - Jurkat

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - K562

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SKOV3

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Human - BMDM

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.