Protein Expression Eukaryotic cells Leukocytes extracts

Get tips on using NeuroMag to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

Get tips on using Autophagy Detection Kit to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using Iscove’s Modified Dulbecco’s Medium to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media HSG cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - K562 cells

Get tips on using Dynabeads™ CD34 Positive Isolation Kit to perform Cell Isolation CD34+ cells

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?