siRNA / miRNA gene silencing Human CRL-5915

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using NucleoSpin® RNA/Protein to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells

Get tips on using Leukocyte Alkaline Phosphatase Kit to perform Acid phosphatase assay cell type - human periodontal ligament cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human osteoblasts

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using Lipofectamine™ 3000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using Quick-RNA™ MiniPrep Plus to perform RNA isolation / purification Cells - primary human epithelial cells

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.