Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries
Get tips on using EpiTect MSP Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MEG3
Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse liver tissue
Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse hippocampal tissue
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - Mouse white adipose tissue
Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid
Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - Rat whole pituitary glands PROP1
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