Immunohistochemistry Estrogen receptor (ER) Rabbit Human

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LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells Product

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit Product

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Products Cosmo Bio GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit

GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit Product

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Products Cosmo Bio GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit

Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay Product

Get tips on using Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Products Thermo Fisher Scientific Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay

Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay Product

Get tips on using Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Products Thermo Fisher Scientific Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay

Agilent DNA 1000 Kit Bioanalyzer DNA Analysis Part Number:5067-1504 Product

Get tips on using Agilent DNA 1000 Kit Bioanalyzer DNA Analysis Part Number:5067-1504 to perform Cell line authentication Human lung carcinoma cell line NCI-H1299

Products Agilent Technologies Agilent DNA 1000 Kit Bioanalyzer DNA Analysis Part Number:5067-1504

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU Product

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Products Thermo Fisher Scientific APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

ChIP Anti-bodies ERα Experiment

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Proteins ChIP Anti-bodies ERα

PL_MmEro1l Product

Get tips on using PL_MmEro1l to perform Protein Expression Eukaryotic cells - CHO Ero1l

Products Helene Faustrup Kildegaard, The Novo Nordisk Foundation Center f PL_MmEro1l

pMJS205 Product

Get tips on using pMJS205 to perform Protein Expression Prokaryotic cells - E. coli S. cerevisiae SOX Erv1p

Products Robyn Roth, Biosciences, Council for Scientific and Industrial R pMJS205

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