siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Liver

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Liver

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MCF-7

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PC12

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human tracheal epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human skeletal muscle cells