sirna-rnai-mirna-transfection-human-cells

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Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Products Qiagen miRNeasy Mini kit

Get tips on using Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - yeast, Yarrowia lipolytica

Products Thermo Fisher Scientific Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines THP-1

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines KG-1

Proteins Protein expression and purification Mammalian cells HEK 293 HER2 leader peptide

Cell culture media 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Mouse muscle stem cells SPRY1

Get tips on using 300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells) to perform Plasmid Isolation Vibrio parahaemolyticus

Products Favorgen 300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells)

Get tips on using MISSION® shRNA SOX2 Lentiviral Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans SOX2 lentiviral particles

Products Sigma-Aldrich MISSION® shRNA SOX2 Lentiviral Transduction Particles

Get tips on using MISSION® shRNA SOX6 Lentiviral Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans SOX6 lentiviral particles

Products Sigma-Aldrich MISSION® shRNA SOX6 Lentiviral Transduction Particles

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