Get tips on using Rabbit anti-calretinin to perform Immunohistochemistry Mouse - Calb2
Get tips on using Ki67/MKI67 Antibody to perform Immunohistochemistry Mouse - Ki67
Get tips on using Human SOX9 Antibody to perform Immunohistochemistry Mouse - SOX9
Get tips on using IRE1 beta antibody to perform Immunohistochemistry Mouse - IRE1β
Get tips on using Proteome Profiler™ Human Apoptosis Array Kit to perform Apoptosis assay cell type - Array of apoptotic proteins
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin
Get tips on using pVRB2B3 to perform Protein Expression Eukaryotic cells - HEK293 hβ-defensin 2/3
Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex
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