rna-isolation-purification-tissue-mouse-testes

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay mouse - C2C12

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay mouse - L929

Get tips on using Annexin V-FITC Kit, 200 Tests, RUO to perform Necrosis MCF7

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using MycoAlert™ PLUS Mycoplasma Detection Kit (30 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma