siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Cell Meter™ Autophagy Assay Kit *Green Fluorescence to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) BCPAP

Get tips on using LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells to perform Live / Dead assay mammalian cells - K562

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media hLung Mesenchymal Stem Cells (MSCs)

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)