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Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay bacteria - Clostridium sporogenes

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Products Biotium Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?

Discussions Impact of using FBS

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus

Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Products VWR VWR Life Science RiboZol™ RNA Extraction Reagent

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

Products Merck Millipore Live/Dead Double Staining Kit (Merck)

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pneumoniae

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Mycobacterium tuberculosis

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Enterococcus faecalis

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