rna-isolation-purification-tissue-mouse-colon

Get tips on using GeneChip Rhesus Macaque Genome Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Get tips on using Live-Dead cell staining kit (Enzo) to perform Live / Dead assay mammalian cells - human fibroblast tissue

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Get tips on using Anti-NSE, Clone MIG-N3 to perform Immunohistochemistry Mouse - NSE

Get tips on using Anti-Calretinin Antibody, clone 6B8.2 to perform Immunohistochemistry Mouse - Calb2

Get tips on using Anti-CD133 Antibody, clone 13A4 to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-Somatostatin Antibody, clone YC7 to perform Immunohistochemistry Mouse - Somatostatin

Get tips on using Anti-Glucagon Antibody, clone 13D11.33 to perform Immunohistochemistry Mouse - Glucagon

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.