DNA isolation / purification Cells

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay Caco-2

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay Hep G2

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay Hep G2

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay HEK 293T

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using pOPINE to perform Protein expression and purification Bacteria - Escherichia coli medin

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MIA PaCa-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MDA-MB-231

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U-87 MG