ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using Mouse Chitinase 3-like 1 Quantikine ELISA Kit to perform ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Rat - IGF-I

Get tips on using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Rat - IL-1 beta

Get tips on using Rat CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Rat - C-Reactive Protein/CRP