Protein isolation

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary human brain microvascular endothelial cells

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using ISOLATE II RNA Micro Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Cells - Primary cells Pseudomyxoma peritonei (PMP) cells

Get tips on using TRIzol™ Plus RNA Purification Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer