siRNA / miRNA gene silencing Human OV2008 Yap Gene

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells