Protein Expression Prokaryotic cells A. tumefaciens

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma

Get tips on using EZ4U - Cell Proliferation Assay to perform Live / Dead assay mammalian cells - GH3

Get tips on using Autophagy Detection Kit to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - K562 cells

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.