crispr-human-repression-hpv-16-e6

Get tips on using Human PLZF Antibody to perform Immunohistochemistry Mouse - PLZF

Get tips on using Human SOX9 Antibody to perform Immunohistochemistry Mouse - SOX9

Get tips on using Human TAK1 Antibody to perform Western blotting Tak1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Human VEGF ELISA Kit (ab100662) to perform ELISA Human - VEGF

Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1

Get tips on using Human RBP4 Quantikine ELISA Kit to perform ELISA Human - RBP4

Get tips on using Human Prolactin/PRL ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin ELISA Kit (ab100618) to perform ELISA Human - OPN