Protein expression and purification Mammalian cells HEK 293

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Rat cell lines

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse cardiac fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat hippocampal neurons