shRNA gene silencing Human Islets of langerhans Negative control (scrambled)

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

Get tips on using ISOGEN to perform RNA isolation / purification Cells - primary human epidermal melanocytes

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human esophageal organoids

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human preadipocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Thyroid gland