Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HL-60
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HL-1
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - CaCo-2
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - SH-SY5Y
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HEK 293
Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19
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