Stem cell culture media

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media U937

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media PNT1A

Get tips on using Corning® 500 mL MEM (Minimum Essential Medium) Alpha Medium to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media THP-1

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media BHK-21

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Choroid plexus-like tissue generation from SFEBq

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria