Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)
Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human prenatal cardiac progenator cells
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - PC-9
Get tips on using Qproteome FFPE Tissue Kit (20) to perform Protein isolation Tissue - Human tissue C-MFPE samples
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment