siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

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Protein isolation Bacteria - Bacillus anthracis Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Bacteria Bacillus anthracis

NucleoSpin® RNA XS Product

Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin

Products Macherey Nagel NucleoSpin® RNA XS

RNeasy Plus Mini Kit Product

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Skin

Products Qiagen RNeasy Plus Mini Kit

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human skeletal muscle cells

Products Thermo Fisher Scientific TRIzol Reagent

Senescence β-Galactosidase Staining Kit - Cell Signaling Product

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - H9C2