rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Rat - HSP70

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Rat - GDNF

Get tips on using 17β-Estradiol ELISA kit to perform ELISA Rat - Estradiol

Get tips on using Rn_LOC311846_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - Astrocytes LRRC8A

Get tips on using Rn_Sod2_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - PC12 SOD2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Human Endometrial Stromal cells Biotin

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA, rhesus monkey brain tissue Human endothelail stromal cells

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.