CRISPR Mouse Deletion RAW 264.7

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF-7

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - SH-SY5Y

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - Hep G2

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - AGS cell line

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.