rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary porcine primary chondrocytes

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human preadipocytes

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using ZR RNA MiniPrepTM kit to perform RNA isolation / purification Cells - primary human endothelial cells