Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Hep3B

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HepG2

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HeLa

Get tips on using GeneBLAzer In Vivo Detection Kit to perform Reporter gene assay β-lactamase substrates - HeLa

Get tips on using pcDNA™3.1D/V5-His TOPO®-hsEH to perform Protein Expression Eukaryotic cells - HEK293 hsEH

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HeLa cells

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - HEK293

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - HeLa

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - HeLa cervical cancer cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.