siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat aortic smooth muscle cells

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat tendon-derived stem cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Live-Dead cell staining kit (Enzo) to perform Live / Dead assay mammalian cells - human fibroblast tissue

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - U2OS (human Bone Osteosarcoma Epithelial Cells)

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - UMR-106 BMP2