Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Kidney
The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.
Get tips on using RNeasy PowerPlant Kit (50) to perform RNA isolation / purification Plants - Seeds
Get tips on using RNeasy PowerWater Kit (50) to perform RNA isolation / purification Water samples
Get tips on using RNeasy 96 Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification Ribonucleoprotein
Get tips on using DyeEx 96 Kit (24) to perform Removal of unincorporated dye terminators
Get tips on using DNeasy PowerWater Kit (100) to perform DNA isolation / purification Water samples
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