DNA transfection Mammalian cells Immortalized cell lines

Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral

Get tips on using Anti-Clara Cell Secretory Protein Antibody to perform Flow cytometry Anti-bodies Mouse - CCSP

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Get tips on using CytoTune™-iPS 2.0 Sendai Reprogramming Kit to perform Stem cell Differentiation media Differentiation of RPE cells into hiPSC cells

Get tips on using EBMTM Endothelial Cell Growth Basal Medium, 500 mL to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using miRCURY Exosome Cell/Urine/CSF Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

Get tips on using ChargeSwitch™ Total RNA Cell Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae

Get tips on using ChargeSwitch™ Total RNA Cell Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.