Immunohistochemistry Collagen Type I Goat

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Anti-Integrin α2 Antibody to perform Western blotting integrin alpha2

Get tips on using siRNA ICAM1 to perform siRNA / miRNA gene silencing Human - HT-1080 ICAM1

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Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Rat Pancreas

Get tips on using MicroRNA isolation kit to perform RNA isolation / purification Yeast - Saccharomyces cerevisiae

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