DNA quantification Mouse

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Medium 231 to perform Mammalian cell culture media DAOY

Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Daudi

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Daoy

Get tips on using siRNA MXD3 to perform siRNA / miRNA gene silencing Human - DAOY MXD3

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Daudi

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Daoy

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Daoy