Immunohistochemistry Vimentin Rabbit Human / mouse

Get tips on using A2B5 Antibody, anti-human/mouse/rat, APC to perform Flow cytometry Anti-bodies Human - A2B5

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal to perform ChIP Anti-bodies H3K4me3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using PE Mouse Anti-Human CD31 Clone L133.1 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Purified anti-mouse/rat/human FOXP3 Antibody to perform Western blotting FOXP3

Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488

Get tips on using Human CRISP-3 Antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26