siRNA / RNAi /miRNA transfection Human Primary splenocytes

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Retina

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brainstem

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Mouse - Chondrocytes

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Pineal gland

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.