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RNA quantification

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Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - C57BL/6

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Products Cell Signaling Technology Atg5 (D5F5U) Rabbit mAb

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Products Cell Signaling Technology Atg12 (D88H11) Rabbit mAb

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - THP 1

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1

Products Cell Signaling Technology SQSTM1/p62 (D5E2) Rabbit mAb

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type RAW 264.7

Get tips on using Polyclonal Rabbit Anti-Human Myeloperoxidase (Dako Omnis) to perform Immunohistochemistry Mouse - MPO

Products Agilent Technologies Polyclonal Rabbit Anti-Human Myeloperoxidase (Dako Omnis)

Get tips on using Anti-UCP-1 antibody produced in rabbit to perform Immunohistochemistry Mouse - UCP1

Products Sigma-Aldrich Anti-UCP-1 antibody produced in rabbit

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