Protein expression and purification Insect cells Hi5

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Chlamydia pneumoniae

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Qproteome Mitochondria Isolation Kit to perform Protein enrichment Mitochondria

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Bacillus cellulosilyticus

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Synechocystis

Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - T47D