Site Directed Mutagenesis (SDM) Mouse Point mutation

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF-7

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - SH-SY5Y

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - Hep G2

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - AGS cell line

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?