Protein expression and purification Mammalian cells HEK 293

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media A431

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media A2780

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) to perform Mammalian cell culture media A253

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media A253

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media A172

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MDCK

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Mammalian cell culture media DH82

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media DH82

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media Vero

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.