rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using EasySep™ Human CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA, rhesus monkey brain tissue Biotin

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 HER2 leader peptide

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - mouse iPSC

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - HLE-B3

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Brain tissue from rhesus monkey Biotin

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.