siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - BJ

Get tips on using JAM-A CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Deletion F11R

Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin

Get tips on using Cell Cycle Phase Determination Kit to perform Cell cycle assay human - AGS cell line

Get tips on using Androgen Receptor (D6F11) XP® Rabbit mAb #5153 to perform Immunohistochemistry Human - AR

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - SW480

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - AGS

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human MEWO

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human A375