DNA methylation profiling Gene specific profiling HEK 293T

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using pFRT to perform Protein Expression Eukaryotic cells - HeLa R19 cMBL

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using AcP(−)p6.9hEPO to perform Protein Expression Eukaryotic cells - S. frugiperda hEPO

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HepG2

Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM

Get tips on using Medium 254CF, Kit to perform Mammalian cell culture media HEM