DNA isolation / purification Cells Immortalized cell lines

Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Qproteome Cell Compartment Kit to perform Protein enrichment Total nuclear proteins

Get tips on using MEGM-Mammary-Epithelial-Cell-Growth-Medium to perform Stem cell culture media hMammospheres

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Get tips on using Canine Smooth Muscle Cell Growth Medium to perform Mammalian cell culture media CnAOSMC

Get tips on using Bovine Brain Endothelial Cell Growth Medium to perform Mammalian cell culture media BBMVEC

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - C2C12

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - L929

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87