ChIP acH4 Mouse Rat

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Get tips on using FITC Rat Anti-Human CD49f to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using ON-TARGETplus Rat Prkaa1 (65248) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Ampkα1/Prkaa1

Get tips on using ON-TARGETplus Rat Prkaa2 (78975) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Ampkα2/Prkaa2

Get tips on using ON-TARGETplus Rat Arhgef6 (363509) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 α-Pix/Arhgef6

Get tips on using ON-TARGETplus Rat Arhgef7 (114559) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 β-Pix/Arhgef7

Get tips on using ON-TARGETplus Rat Itgb1 (24511) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 β1 integrin/Itgb1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Get tips on using ON-TARGETplus Rat Rac1 (363875) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - MTLn3 Rac1