siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT

Get tips on using β-Galactosidase Staining Kit to perform Reporter gene assay β-galactosidase substrates - human knee bone tissue

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Whole blood (human) FKBP5

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Insulin (Autostainer Link 48) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Insulin (C27C9) Rabbit mAb #3014 to perform Immunohistochemistry Mouse - Insulin

Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62