siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - RCC

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - PBMC

Get tips on using ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA to perform ELISA Human - GDNF

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media Brain organoids from Human iPSCs

Get tips on using Cell Cycle Phase Determination Kit to perform Cell cycle assay human - AGS cell line

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HT-29