DNA transfection Mammalian cells Primary cells

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DNA DNA isolation / purification Cells Immortalized cell lines MKN45

Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid

Products Mirus TransIT®-LT1 Transfection Reagent

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Products Qiagen DNeasy Blood & Tissue Kit

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling HCT116, HTC15 human colon cancer cells

Get tips on using TransMessenger Transfection Reagent (0.5 ml) to perform siRNA / RNAi /miRNA transfection Mouse - Primary cortical and hippocampal cell

Products Qiagen TransMessenger Transfection Reagent (0.5 ml)

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells CD4+ T cells

Products Qiagen Gentra Puregene Blood Kit

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Products Qiagen DNeasy Blood & Tissue Kit

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Products Qiagen AllPrep DNA/RNA Mini Kit

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Products Qiagen AllPrep DNA/RNA Mini Kit

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