Site Directed Mutagenesis (SDM) Mouse Point mutation 3T3-L1

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The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Rat Deletion INS-1 832/13 Ep300

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Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

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Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1

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Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL

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Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

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Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

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Get tips on using Donkey anti-mouse IgG to perform Immunohistochemistry Anti-mouse IgG - Donkey Mouse Rhodamin red

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized NIH-3T3

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