Western blot p62/SQSTM1 Mouse IgG2b

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Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL

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Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

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Get tips on using Biotin Rat Anti-Mouse CD45 to perform Flow cytometry Anti-bodies Mouse - CD45

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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Mouse aortic endothelial cells (MAECs)

Get tips on using Biotin Rat Anti-Mouse CD106 to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

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Get tips on using Biotin anti-mouse CD106 Antibody to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

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Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

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Get tips on using Donkey anti-mouse IgG to perform Immunohistochemistry Anti-mouse IgG - Donkey Mouse Rhodamin red

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Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34

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Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

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