DNA isolation / purification Cells Immortalized cell lines

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media HPAEC

Get tips on using Dead Cell Apoptosis Kit with Annexin V FITC and PI to perform Apoptosis assay cell type - OECM-1

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils

Get tips on using MTT Cell Proliferation Assay (ATCC® 30-1010K™) to perform Cell cytotoxicity / Proliferation assay cell type - MCF-7

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media Mice BMSC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media CADMEC/HMVEC

Get tips on using X-VIVOTM 10 Serum-free Hematopoietic Cell Medium to perform Mammalian cell culture media NK-92

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Torin 1 to perform Autophagy assay cell type - Mel cells